monarch gel dissolving buffer (New England Biolabs)
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monarch gel dissolving buffer
Monarch Gel Dissolving Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/monarch+gel+dissolving+buffer/Monarch+Buffer+BY/pmc11551759-42-11-15
Average 95 stars, based on 48 article reviews
Monarch Gel Dissolving Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/monarch+gel+dissolving+buffer/Monarch+Buffer+BY/pmc11551759-42-11-15
Average 95 stars, based on 48 article reviews
monarch gel dissolving buffer - by Bioz Stars,
2026-09
95/100 stars
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Purification:Article Title: The interplay between peptides and RNA is critical for protoribosome compartmentalization and stability Article Snippet: Then, 1 μL of 5U/ μL Klenow Fragment of DNA polymerase I (NEB) and 2 μL of 10 mM dNTP mix (SERVA) were added to the reaction mixture, and the reaction mixture was kept at 25°C for 1 hour followed by heat inactivation of enzyme at 50°C for 15 min. For the synthesis of Sh1 and Sh2 sPTC rRNAs, 40 pmol of Sh1_sPTC_R / Sh2_sPTC_R ssDNA and T7_F ( Supplementary Table 1 ) was mixed in H 2 O to the total volume of 36 μl, incubated 95°C for 1 min and then kept at room temperature for 2 min; this was used as DNA template (1.9 μg of DNA in total). .. The resulting dsDNA templates were purified from 2% TAE-agarose gel using Article Title: The interplay between peptides and RNA is critical for protoribosome compartmentalization and stability. Article Snippet: Then, 1 μl of 5U / μl Klenow Fragment of DNA polymerase I (NEB) and 2 μl of 10 mM dNTP mix (SERVA) were added to the reaction mixture, and the reaction mixture was kept at 25 ◦C for 1 h followed by heat inactivation of enzyme at 50 ◦C for 15 min. For the synthesis of Sh1 and Sh2 sPTC rRNAs, 40 pmol of Sh1_sPTC_R / Sh2_sPTC_R ssDNA and T7_F ( Supplementary Table S1 ) was mixed in H 2 O to the total volume of 36 μl, incubated at 95 ◦C for 1 min and then kept at room temperature for 2 min; this was used as DNA template (1.9 μg of DNA in total). .. The resulting dsDNA templates were purified from 2% (Tris-acetate-EDTA)-agarose gel using Article Title: The interplay between peptides and RNA is critical for protoribosome compartmentalization and stability Article Snippet: Then, 1 μl of 5U/μl Klenow Fragment of DNA polymerase I (NEB) and 2 μl of 10 mM dNTP mix (SERVA) were added to the reaction mixture, and the reaction mixture was kept at 25°C for 1 h followed by heat inactivation of enzyme at 50°C for 15 min. For the synthesis of Sh1 and Sh2 sPTC rRNAs, 40 pmol of Sh1_sPTC_R/Sh2_sPTC_R ssDNA and T7_F ( ) was mixed in H 2 O to the total volume of 36 μl, incubated at 95°C for 1 min and then kept at room temperature for 2 min; this was used as DNA template (1.9 μg of DNA in total). .. The resulting dsDNA templates were purified from 2% (Tris-acetate-EDTA)-agarose gel using Polymerase Chain Reaction:Article Title: The interplay between peptides and RNA is critical for protoribosome compartmentalization and stability Article Snippet: Then, 1 μL of 5U/ μL Klenow Fragment of DNA polymerase I (NEB) and 2 μL of 10 mM dNTP mix (SERVA) were added to the reaction mixture, and the reaction mixture was kept at 25°C for 1 hour followed by heat inactivation of enzyme at 50°C for 15 min. For the synthesis of Sh1 and Sh2 sPTC rRNAs, 40 pmol of Sh1_sPTC_R / Sh2_sPTC_R ssDNA and T7_F ( Supplementary Table 1 ) was mixed in H 2 O to the total volume of 36 μl, incubated 95°C for 1 min and then kept at room temperature for 2 min; this was used as DNA template (1.9 μg of DNA in total). .. The resulting dsDNA templates were purified from 2% TAE-agarose gel using Article Title: The interplay between peptides and RNA is critical for protoribosome compartmentalization and stability. Article Snippet: Then, 1 μl of 5U / μl Klenow Fragment of DNA polymerase I (NEB) and 2 μl of 10 mM dNTP mix (SERVA) were added to the reaction mixture, and the reaction mixture was kept at 25 ◦C for 1 h followed by heat inactivation of enzyme at 50 ◦C for 15 min. For the synthesis of Sh1 and Sh2 sPTC rRNAs, 40 pmol of Sh1_sPTC_R / Sh2_sPTC_R ssDNA and T7_F ( Supplementary Table S1 ) was mixed in H 2 O to the total volume of 36 μl, incubated at 95 ◦C for 1 min and then kept at room temperature for 2 min; this was used as DNA template (1.9 μg of DNA in total). .. The resulting dsDNA templates were purified from 2% (Tris-acetate-EDTA)-agarose gel using Article Title: The interplay between peptides and RNA is critical for protoribosome compartmentalization and stability Article Snippet: Then, 1 μl of 5U/μl Klenow Fragment of DNA polymerase I (NEB) and 2 μl of 10 mM dNTP mix (SERVA) were added to the reaction mixture, and the reaction mixture was kept at 25°C for 1 h followed by heat inactivation of enzyme at 50°C for 15 min. For the synthesis of Sh1 and Sh2 sPTC rRNAs, 40 pmol of Sh1_sPTC_R/Sh2_sPTC_R ssDNA and T7_F ( ) was mixed in H 2 O to the total volume of 36 μl, incubated at 95°C for 1 min and then kept at room temperature for 2 min; this was used as DNA template (1.9 μg of DNA in total). .. The resulting dsDNA templates were purified from 2% (Tris-acetate-EDTA)-agarose gel using Incubation:Article Title: Co-transcriptional production of programmable RNA condensates and synthetic organelles Article Snippet: One PCR reaction (50 μl + 10 μl TriTrack DNA Loading Dye 6×, Thermo Scientific) was loaded in each well, and bands were cut using a scalpel under the ultraviolet illumination. .. Gel bands were loaded in pairs in 2 ml Eppendorf tubes, treated by adding 4 μl of Article Title: Co-transcriptional production of programmable RNA condensates and synthetic organelles. Article Snippet: One PCR reaction (50 μl + 10 μl TriTrack DNA Loading Dye 6×, Thermo Scientific) was loaded in each well, and bands were cut using a scalpel under the ultraviolet illumination. .. Gel bands were loaded in pairs in 2 ml Eppendorf tubes, treated by adding 4 μl of Dissolution:Article Title: Co-transcriptional production of programmable RNA condensates and synthetic organelles Article Snippet: One PCR reaction (50 μl + 10 μl TriTrack DNA Loading Dye 6×, Thermo Scientific) was loaded in each well, and bands were cut using a scalpel under the ultraviolet illumination. .. Gel bands were loaded in pairs in 2 ml Eppendorf tubes, treated by adding 4 μl of Article Title: Co-transcriptional production of programmable RNA condensates and synthetic organelles. Article Snippet: One PCR reaction (50 μl + 10 μl TriTrack DNA Loading Dye 6×, Thermo Scientific) was loaded in each well, and bands were cut using a scalpel under the ultraviolet illumination. .. Gel bands were loaded in pairs in 2 ml Eppendorf tubes, treated by adding 4 μl of |